Study on the Free and Pyrolyzed Hydrocarbons of Bacillus. Subtilis
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摘要: 采用气相色谱和色谱－质谱联用仪对枯草杆菌（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）游离烃类及热解烃类进行了分析。枯草杆菌热解产气率较高（６７．８ｍｌ／ｇ），正异构比值较低，乙烷乙烯比值较高。热解作用使氯仿抽提物含量大幅度提高，达热解前的１０．７４倍。枯草杆菌游离烷烃以正烷烃为主，主峰为正二十七烷，碳数分布范围为Ｃ16－Ｃ30。热解产物中烷烃以Ｃ12－Ｃ20支链烷烃、Ｃ162－Ｃ25正烷烃和Ｃ15－Ｃ20类异成二烯烃为主，主峰为３－甲基十三烷，正烷烃以Ｃ15含量最高。文章还讨论了热解作用提高枯草杆菌氯仿抽提物含量，改变烃类分布的可能原因及意义。Abstract: The free hydrocarbons and vacuum pyrolyzed hydrocarbons of B. subtilis were analyzed by GC and GCMS system. The gas generation rate in B.subtilis during thermal degradation was high (67. 8ml/g), the ratio of normal and isomeric alkanes was low, and that of ethane to ethylene was high. The content of cholroform extractable organic matter increased by 9. 74 times after thermal degradation. The free hydrocarbons were mainly composed of C16-C30 n -alkanes with C27 as a main peak. The pyrolyzed hydrocarbons were mainly composed of C12-C20 branched-chain alkanes, C12-C25 n-alkanes and C15-C20, isoprenoid alkanes, with 3-Methyltridecane as a main peak, the content of C15 n-alkane was the highest among all n-alkanes.
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Key words:
- B. subtilis /
- Hydrocarbon
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